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Ribosome profiling of Rbfox1 fl/fl-Cre and control cortical culture

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NIAID Data Ecosystem2026-05-02 收录
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The goal of this experiment is to analyze differences in translation of mRNA transcripts in absence vs presence of Rbfox1 using ribosome profiling of Rbfox1-cKO and control mature primary neuronal cultures Overall design: E15 cortical cultres from a Rbfox1 fl/fl pregnant mouse were plated in 10cm plates, cells were infected with AAV-hSyn-Cre-eGFP or AAV-hSyn-eGFP at DIV3, harvested cells at DIV14. Two technical replicates per sample type

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2024-05-16
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