Subclone frequency estimates are only accurate at detectable frequency ranges (~10–45% VAF).

(Left) The distribution of predicted subclone frequencies when one subclone was correctly predicted (true) vs incorrectly predicted (false). (Right) True subclone frequency (as defined by TEMULATOR) versus predicted subclone frequency. Note that a subclone must appear between ~10–40% VAF for accurate estimation. Blue dots indicate correct prediction of selection. Red dots indicate incorrect prediction of neutrality. (ZIP)