Effects of transient thermal stress on thyroid cancer cells

Radiofrequency ablation has been used to treat low-risk thyroid cancer; however, its oncological safety remains unclear. We applied transient sublethal thermal stress to thyroid cancer cells to simulate clinical scenarios in which incomplete radiofrequency ablation leads to residual viable thyroid cancer cells. Overall design: Thyroid cancer cell lines TPC-1 and B-CPAP were grown in T25 flasks submerged at 37 or 40 degrees Celsius for 20 minutes. Total RNA was extracted and subjected to RNA-seq.