Ceramides drive colorectal cancer metastasis through a revival stemness program [scRNA-seq]

We demonstrate that tumor-intrinsic ceramide metabolism is crucial in initiating colorectal cancer (CRC) metastasis, a process further enhanced by dietary fat intake Overall design: Alive cells were isolated from normal colons or primary colon tumors of high fat diet-fed samples and the matched control samples by fluorescence-activated cell sorting (FACS) and then scRNAseq experiments and analyses were performed.