The role of extracellular vesicle miRNAs and tRNAs in synovial fibroblast senescence

We studied extracellular vesicles (EVs) from synovial fibroblast (SF). EVs were isolated from the secretome of non-senescent and irradiation-induced senescent SFs using size exclusion chromatography. EV RNA was extracted and subject to small RNA sequencing on an Illumina NovaSeq SP using 100bp, single end reads. After mapping against GRCh38.p12 and miRBase v22.1 differential expression analysis was undertaken with edgeRv3.28 using a quasi-likelihood negative binomial generalized log-linear model. We identified a panel of differentially expressed small non-coding RNAs. Overall design: Examination of small non-coding RNAs of extracellular vesiscles derived form the secretome of synovial fibroblasts from osteoathritic joint tissue using pairwise comparisons.