Long noncoding RNA GATA2AS regulates human erythroid differentiation by controlling erythroid transcription factors binding and regulatory landscape. Long noncoding RNA GATA2AS regulates human erythroid differentiation by controlling erythroid transcription factors binding and regulatory landscape

To study how GATA2AS regulates erythroid differentiation, GATA2AS ChIRP-seq, RNA-seq, LRF and KLF1 ChIP-seq, H3K27ac ChIP-seq and ATAC-seq were performed. Overall design: GATA2AS ChIRP-seq was performed in wild type Hudep2 cells. RNA-seq were performed in KO control and GATA2AS KO Hudep2 cells on day 0/4/7/9 during differentiation and in KD control and GATA2AS KD CD34 cells on day 6/10/14 during differentiation. LRF and KLF1 ChIP-seq were performed in KO control and GATA2AS KO Hudep2 cells to investigate how GATA2AS affects LRF and KLF1 binding. H3K27ac ChIP-seq and ATAC-seq were performed in KO control and GATA2AS KO Hudep2 cells to study how GATA2AS affects regulatory landscape.