Developing a cryopreservation protocol for the forest-forming seaweed Phyllospora comosa as a tool for restoration

Global declines in seaweed forests are reducing genetic diversity and, consequently, the resilience of these habitats to changing environmental conditions. To conserve genetic diversity and secure these important coastal habitats, new preservation strategies are needed, such as cryopreservation. We aimed to develop a cryopreservation protocol for sperm and germlings of the fucoid seaweed Phyllospora comosa, a species highly vulnerable to anthropogenic stressors. We tested the cytotoxicity of cryoprotectant agents commonly used in seaweed cryopreservation (dimethyl sulfoxide, ethylene glycol, glycerol, methanol and propylene glycol). We conducted seven experiments that are related to individual datasets. All data were collected from these experiments. All datasets were analysed utilising the same R code and details can be found in the README file.全球海藻林的衰退正导致其遗传多样性下降，进而降低这些沿岸生境应对环境变化的恢复力。为保护遗传多样性并保全这些重要的沿岸生境，亟需开发新型保存策略，例如超低温保存（cryopreservation）。本研究旨在为墨角藻目海藻*Phyllospora comosa*（该物种对人为胁迫因子具有高度易感性）的精子与幼孢子体开发一套超低温保存方案。我们测试了海藻超低温保存中常用的抗冻保护剂（cryoprotectant agents）的细胞毒性，包括二甲基亚砜（dimethyl sulfoxide）、乙二醇（ethylene glycol）、甘油（glycerol）、甲醇（methanol）以及丙二醇（propylene glycol）。 本研究共开展七项分别对应独立数据集的实验，所有数据均采集自上述实验。所有数据集均采用同一套R代码进行分析，详细信息可在README文件中查阅。