Genome assemblies and annotation of <i>Verpa bohemica </i>and <i>Verpa conica</i>

We constructed subchromosomal genome assemblies for six strains of <i>Verpa </i>combing Nanopore and Illumina sequencing technologies. The average assembly sizes were 44.38 Mb for <i>V. bohemica</i> and 45.40 Mb for <i>V. conica</i>. Additionally, Hi-C data were used to anchor <i>V. bohemica</i> strain 21108 and <i>V. conica</i> strain 21120 to chromosomes 26 and 25, respectively. Benchmarking Universal Single-Copy Orthologue (BUSCO) analysis revealed that the completeness of the final assemblies exceeds 95%. Following the assembly and quality assessments, we annotated protein-coding genes and analyzed the phylogenetic relationships of the genus <i>Verpa</i>. We observed the presence of multiple accessory chromosomes in <i>Verpa</i>, suggesting the complexity of their chromosomal evolution. Furthermore, trough analyses of expaned gene families, along with the detecting of carbohydrate-active enzymes (CAZymes), we conducted a preliminary exploration of the genetic basis underlying the saprotrophic nutritional strategy of <i>Verpa</i>.