Hemolytic activity of TRH variants and TDH2 expressed in a cell-free system.

Approx. 300 ng of soluble protein were combined with 4% erythrocyte suspension of each blood type respectively. Hemolytic activity was determined by spectro-photometrical measurement at OD570 and set in proportion (%) to the maximum hemolysis in the positive control (100%). m = mature protein (without signal peptide) Hemolytic activity of TRH variants and TDH2 expressed in a cell-free system.