Effect of curcumin on prostaglandin E2, matrix metalloproteinase-3 and proteoglycan release in articular cartilage

Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and no curcumin (control). Green staining (calcein AM) indicates live metabolizing cells.
Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and 12.5 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells.
Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and 25 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells.
Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 10ng/ml IL-1β, 2% penicillin/streptomycin and 100 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells.
Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and 100 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells.
Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and sodium nitroprusside (positive control). Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells.
Cytotoxicity values of various compounds on equine chondrocytes. Values refer to percentage of dead cells counted. SNP – sodium nitroprusside; NSAID - carprofen; DMSO - dimethyl sulfoxide. Micromolar values refer to curcumin concentrations. Days refer to the time cell death was measured after compound exposure.
Proteoglycan (GAG) and supernatant release (uM/ml) of equine cartilage explants in response to various compounds. SNP – sodium nitroprusside; IL-β – interleukin beta. Micromolar values refer to curcumin concentrations.
Quantification of matrix metalloproteinase (MMP-3) blots in three different samples of equine cartilage explant secretome (culture supernatants).
Effect of various compounds on proteoglycan (GAG) release (uM/ml) in three equine cartilage explants. SNP – sodium nitroprusside; NSAID - carprofen; DMSO - dimethyl sulfoxide. Micromolar values refer to curcumin concentrations.
Effect of various compounds on prostaglandin (PGE) and proteoglycan (GAG) release (uM/ml) in three IL-β stimulated equine cartilage explants. SNP – sodium nitroprusside; NSAID - carprofen; DMSO - dimethyl sulfoxide. Micromolar values refer to curcumin concentrations.

通过马软骨切片（15μm厚度）进行三维共聚焦重建的z栈，培养五日后。细胞在含有2%青霉素/链霉素和不含姜黄素（对照组）的培养基中培养。绿色染色（钙黄绿素AM）表明活代谢细胞。三维共聚焦重建的z栈通过马软骨切片（15μm切片）培养五日后。细胞在含有2%青霉素/链霉素和12.5 µM姜黄素的培养基中培养。绿色染色（钙黄绿素AM）表明活代谢细胞，红色染色（乙二胺二甲基苯并咪唑-1）突出死细胞的核。三维共聚焦重建的z栈通过马软骨切片（15μm切片）培养五日后。细胞在含有2%青霉素/链霉素和25 µM姜黄素的培养基中培养。绿色染色（钙黄绿素AM）表明活代谢细胞。三维共聚焦重建的z栈通过马软骨切片（15μm切片）培养五日后。细胞在含有2%青霉素/链霉素和10ng/ml IL-1β、2%青霉素/链霉素和100 µM姜黄素的培养基中培养。绿色染色（钙黄绿素AM）表明活代谢细胞，红色染色（乙二胺二甲基苯并咪唑-1）突出死细胞的核。三维共聚焦重建的z栈通过马软骨切片（15μm切片）培养五日后。细胞在含有2%青霉素/链霉素和100 µM姜黄素的培养基中培养。绿色染色（钙黄绿素AM）表明活代谢细胞，红色染色（乙二胺二甲基苯并咪唑-1）突出死细胞的核。三维共聚焦重建的z栈通过马软骨切片（15μm切片）培养五日后。细胞在含有2%青霉素/链霉素和亚硝基铁氰化钠（阳性对照）的培养基中培养。绿色染色（钙黄绿素AM）表明活代谢细胞，红色染色（乙二胺二甲基苯并咪唑-1）突出死细胞的核。各种化合物对马软骨细胞毒性的评价。值指死细胞计数的百分比。SNP - 亚硝基铁氰化钠；NSAID - 水杨酸类；DMSO - 二甲基亚砜。微摩尔值指姜黄素浓度。天数指化合物暴露后细胞死亡测量时间。马软骨切片对各种化合物响应的蛋白聚糖（GAG）和上清液释放（uM/ml）。SNP - 亚硝基铁氰化钠；IL-β - 白细胞介素β。微摩尔值指姜黄素浓度。马软骨切片分泌物（培养上清液）中三种不同样本的基质金属蛋白酶（MMP-3）印迹定量。各种化合物对三种马软骨切片蛋白聚糖（GAG）释放（uM/ml）的影响。SNP - 亚硝基铁氰化钠；NSAID - 水杨酸类；DMSO - 二甲基亚砜。微摩尔值指姜黄素浓度。各种化合物对前列腺素（PGE）和蛋白聚糖（GAG）释放（uM/ml）在三种IL-β刺激的马软骨切片的影响。SNP - 亚硝基铁氰化钠；NSAID - 水杨酸类；DMSO - 二甲基亚砜。微摩尔值指姜黄素浓度。