CD4 down-regulation by nef alleles isolated from human immunodeficiency virus type 1-infected individuals.
收藏PubMed Central1993-06-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC46758/
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资源简介：
PCR was used to clone isolates of the human immunodeficiency virus type 1 (HIV-1) nef gene directly from peripheral blood leukocytes of HIV-1-infected individuals. A transient expression system with human CEM T cells was used to assess the effect of nef on CD4 antigen expression on the cell surface. We show that CD4 down-regulation is a frequent property of primary HIV-1 nef alleles. Mutations in conserved amino acid motifs of Nef disrupted CD4 down-regulation. Our observations strongly suggest that CD4 down-regulation reflects a conserved function of nef, which is selected in vivo in human HIV-1 infection. Methodology described here provides quantitative assays to establish whether alterations in nef correlate with the dynamics of disease progression in human AIDS. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1993-06-15
