Real-time deformability cytometry reference data

This dataset consists of four exemplary real-time fluorescence and deformability cytometry measurements. The HDF5-files can be opened with dclab [1] or Shape-Out [2].<br><br><b>calibration_beads.rtdc</b><br>The calibartion beads (8 Peaks, PolyAN) consist of eight bead populations with different mixtures of fluorophores.<br><br><br><b>CD34_HSPC.rtdc</b><br>Hematopoietic stem and progenitor cells (HSPCs) were obtained using apheresis. The cells were tagged with a fluorescently labeled antibody that binds to the CD34 transmembrane protein. CD34-positive HSPCs are gated with `fl3_max &gt; 90`. Set `area_ratio &lt; 1.05` to remove aggregates. Data were used in [3].<br><br><br><b>leukocytes.rtdc</b><br>The leukocyte population (white blood cells) of this blood sample can be visualized by setting `aspect &lt; 2` and `area_ratio &lt; 1.05`. For more information, see e.g. [4].<br><br><br><b>reticulocytes.rtdc</b><br>Blood contains mostly red blood cells (RBCs) and about 1% reticulocytes (which develop into mature RBCs). Reticulocytes contain ribosomal RNA which was stained with Syto13 for this measurement. Set `area_ratio &lt; 1.05` to remove aggregates. Data were used in [3].<br><br><br>[1] https://github.com/ZellMechanik-Dresden/dclab<br><br>[2] https://github.com/ZellMechanik-Dresden/ShapeOut<br><br>[3] Rosendahl et al., "Real-time fluorescence and deformability cytometry". Nature Methods, 15(5):355–358, 2018. doi:10.1038/nmeth.4639.<br><br>[4] Toepfner et al., "Detection of human disease conditions by single-cell morpho-rheological phenotyping of whole blood". eLife, 7:e29213, 2017. doi:10.1101/145078.<br><br><br>SHA256 sums:<br>08c2ef13eed903ef0f9e451727ab8484df09b5d3b39227dab726e0164dcbe244 calibration_beads.rtdc<br>663b44a9db88d85996500045489e37a317cf115719223a531d617f8e3d450e79 CD34_HSPC.rtdc<br>68bd538b42ffb990f1db52d5f3b21f37c9aff31208ab284f3910fd6872c40fdb leukocytes.rtdc<br>5c323ea75bf7eeb2a28d922730772d50270dd872d6957e60d6062663f3628fb3 reticulocytes.rtdc