Differential gene expression in IDH1-R132H low grade glioma animal brain tumors brain in response to 20 Gy of radiation. Mus musculus

IDH1-R132H is expressed in Low Grade Glioma (LGG) in combination with loss of function mutation in ATRX and TP53 genes. IDH1-R132H results in gain of function with production of 2-hydroxygluatrate, that in turn generates a hypermethylated phenotype in DNA and histone with consequences in epigenetic regulation of gene expression. Here we will compare the gene expression profile between IDH1-R132H and IDH1 Wt LLG moribund animal brain tumors and identify key genes responsible for the phenotype of this subtype of glioma. Overall design: Evaluate differential gene expression between Brain IDH1-R132H and IDH1 wt in response to 20Gy of ionizing radiation (IR) at time that animals become moribunds.