Data for "Variability of the mechanical properties of a single bone cell population"

Pre-processed data obtained by AFM nano-indentation of MLO-A5 pre-osteocyte cells. The data pre-processing consisted in contact point fitting, tip-sample separation correction and drift correction of raw data, all performed in MATLAB (v2016a).The experimental methodology and data processing are described in the author's thesis (Chapter 3). Methods* AFM cantilever preparationTip-less cantilevers (Windsor Scientific) with nominal spring constant of 0.2 N/m were customised by glueing a silica bead (D = 6 μm, Bangs Laboratories) at the tip extremity. * AFM set-upA NanoWizard 3 atomic force microscope (JPK Instruments AG) coupled to an Eclipse Ti-S optical inverted microscope (Nikon Instruments) was used for all the experiments.The cells were washed in phosphate buffer solution (PBS) and fresh medium was added. Samples were positioned on the heated sample holder to allow for testing at 37◦C. Single cells were located through the coupled optical microscope and images of cell shape were recorded for morphological analysis.The cantilever was centred over the location of interest and a grid of 5 points spaced 3 μm within each other was set. Force spectroscopy measurements were obtained on the 5-point grid for 3 times to collect a total of 15 data on each location. The relative set point and the approach velocity were set to 10 nN and 4 μm/s respectively.

经过原子力显微镜（AFM）对MLO-A5成骨细胞前体细胞进行的预处理数据。数据处理包括接触点拟合、尖端与样品分离校正以及原始数据的漂移校正，所有操作均在MATLAB（v2016a）软件平台上完成。实验方法和数据处理方法详见作者论文（第三章）。

方法：
* AFM悬臂制备：采用Windsor Scientific生产的无尖端悬臂（弹簧常数约为0.2 N/m），通过在尖端末端粘贴一个二氧化硅微球（直径为6 μm，Bangs Laboratories）进行定制。
* AFM实验装置：使用JPK Instruments AG生产的NanoWizard 3原子力显微镜，与Nikon Instruments的Eclipse Ti-S光学倒置显微镜耦合，用于所有实验。细胞用磷酸盐缓冲溶液（PBS）清洗，并添加新鲜培养基。样品放置在加热样品台上，以便在37◦C下进行测试。通过耦合的光学显微镜定位单个细胞，并记录细胞形态图像以进行形态学分析。将悬臂中心对准感兴趣的位置，并设置一个由间距为3 μm的5个点组成的网格。在5点网格上进行3次力谱测量，以收集每个位置的15个数据点。相对设定点和接近速度分别设置为10 nN和4 μm/s。