Frequency and potency of binders derived from murine diversity.

Information is provided on the MnA, MiB and MiC sequences of the most amplified VH after three rounds of selection against hIFNγ according to NGS with the corresponding percentages (from 0.3%). Most of clones with identical VH have been found by sequencing random clones with the Sanger method (referred as “found in screening”). Some others which had not been found were rescued by PCR from the output of selection. Information on VL sequences is then also described. The clones were purified as scFv and tested in dose response ELISA against hIFNγ, the last column shows the EC50 values observed in nM (n≥2).