Candidate sRNAs validated by Northern blot.

anstSGR group as defined in Table 1: A and B, unique in PAO1 or PA14, respectively; C and E, conserved in both strains but expressed in either PAO1 or PA14, respectively; D, conserved and expressed in both strains.
bName or number (e.g. 2326 is PA2326, 30840 is PA14_30840) of loci in the PAO1 and PA14 genomes either overlapping (class II, 5′-UTR; III, asRNA; and V, sense sRNAs overlapping annotated ORFs) or flanking (class I, sRNA) the nstSGRs.
cAnnotated sRNAs, found by sRNA-Seq, used as a positive control in Northern blot validation experiments.
dUpper (+) or lower (−) genomic DNA strand coincident with cDNA reads.
esRNA size predicted by sRNA-Seq. Single values indicates coherent results between PAO1 and PA14. Otherwise, two values (PAO1-PA14) are reported. Values are indicated in bold whenever confirmed by Northern blotting. MRE: Multiple Read Ends defined by non-overlapping reads scattered within the nstSGR. The name of sRNAs used as validation controls is also indicated.
fClass assignment in PAO1. The corresponding nstSGR in PA14 was assigned to class V.