Development and validation of enzyme-linked immunosorbent assays for the serodiagnosis of canine Bartonelloses

Bartonella species, emerging vector-borne pathogens of dogs, are increasingly associated with severe, chronic sequelae, as well as potentially life-threatening diseases such as endocarditis and myocarditis. Diagnosis of Bartonelloses is mainly based on PCR, culture, and serological assays. Despite molecular and biotechnological advances, serological assays employing Immunofluorescence antibody (IFA), Western Blotting (WB), and enzyme-linked-immunosorbent-assay (ELISA) technologies have encountered diagnostic limitations, primarily due to poor sensitivity. Using sera from Bartonella-infected and naïve dogs, we applied an immunoproteomic approach to develop a reliable ELISA assay for the diagnosis of Bartonelloses in dogs. Five recombinant Bartonella henselae immunodominant proteins (rATP-β, rGroEL, rLemA, rSucB, and rVirB5) were tested in an ELISA format. Dogs comprised Group I: 36 Bartonella spp. naturally infected dogs (all B. henselae IFA seroreactive) and Group II: 34 Bartonella..., , # Development and validation of enzyme-linked immunosorbent assays for the serodiagnosis of canine Bartonelloses

We have submitted our raw data (**Dogs_ELISA_OD_data.csv**), rATP-β analysis (**rATP-β_analysis.csv**), rATP-β plus rGroEL analysis (**rATP-β_plus_rGroEL_analysis.csv**), rGroEL analysis (**rGroEL_analysis.csv**), rLemA analysis (**rLemA analysis.csv**), rSucB analysis (**rSucB_analysis.csv**), and rVirB5 analysis (**rVirB5_analysis.csv**).

[Access this dataset on Dryad](https://doi.org/10.5061/dryad.9zw3r22t9)

In this study, we evaluated the diagnostic utility of B. henselae recombinant proteins by ELISA using sera from 70 dogs (36 Bartonella spp. IFA-positive (naturally infected) and 34 Bartonella spp. IFA-negative and PCR-negative (control dogs). ELISA optical density (OD) values were measured at 450 nm.

The data and analysis files are standalone and can be retrieved or used without any additional dependencies.

### Dogs_ELISA_OD_data.csv

This file contains the immuno...,