Reduced allergic lung inflammation in IL-10 KO mice.

Semiquantitative analysis of histopathological changes. After lavage, lungs were instilled and fixed with 10% buffered formalin. Following paraffin embedding, sections for microscopy were stained with H&E and PAS. An index of pathologic changes in H&E slides was obtained by scoring the inflammatory infiltrate around the airways and vessels for greatest severity (0, normal; 1, 10 cells diameter thick) and overall extent (0, normal; 1, 75%). The Index was calculated by multiplying severity by extent. An histological goblet cell score was obtained in Periodic acid-Schiff (PAS)-stained lung sections by examining 10 to 20 consecutive airways from all groups of mice at 40x magnification and categorized according to the abundance of PAS-positive goblet (0, 75%). The Index was calculated by dividing the sum of the airway scores from each lung by the number of airways examined for the histological goblet cell score. MEAN±ES;*p**pa posteriori.