Pancreatic Progenitors in ARX ko hESCs.
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(A) Immunostaining of PDX1 (green) and NKX6.1 (red) in agarose-embedded wild type (WT) and ARX knockout (ARX ko) cell sheets from day 14, 17, 20, and 26 of culture. * indicates region of enlargement (right) which includes individual red and green channels and overlay with DAPI (blue) showing coexpression patterns. (B) Immunostaining of 13 week human fetal pancreatic tissue the same as A. (C) RT-qPCR of day 17 and day 26 whole population samples examining the expression of transcription factors believed to be involved in pancreatic progenitor induction. Wild type (black bars) and ARX ko clone 1 (red bars) cell samples relative to adult human islets expression levels. † indicates p
创建时间:
2016-02-22



