Assembly of pGFPi from 10 plasmids.
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Restriction-ligation was performed with continuous incubation at 37°C or for 25 or 50 cycles (2 min 37°C+5 min 16°C). 20 µl out of 1 ml were plated. The negative control was performed without BsaI enzyme.
创建时间：
2009-05-14
