Choroid Plexus Gene Expression in wild-type (wt) and ApoE-deficient Mouse Choroid Plexuses

Laser capture microdissected choroid plexuses were obtained and expression arrays were generated to investigate gene expression in wt and ApoE choroid plexuses; the choroid plexus forms the cerebrospinal fluid, the cerebrospinal fliod barrier, functions as the major gateway for blood-born leukocytes to enter the brain in degenerative and inflammatory brain diseases, and the principal neuroimmune interface in the brain. We found lipid deposits in the aged choroid plexus of hyperlipidemic mice but none in the wt control choroid plexuses. Here, we studied the functional impact and gene epressions in wt and ApoE-deficient choroid plexuses. wt and ApoE mice were maintained on normal mouse chow and allowed to age for 78 weeks; then, choroid plexuses were obtained by laser capture microdissection; total RNA was prepared and whole genome microarrays generated