General outcomes per stage of treatment.

Overview of total number embryos treated, survival at 5 dpf, the presence of morphological abnormalities at 5 dpf, and the degree of severity of those abnormalities.Key: n.a., not applicable;*developmental stage [34] at which embryo was exposed to 10% ethanol (or vehicle only) for 1 h;¶, morphology at 5 dpf was classified as normal or abnormal according to the criteria in Table 5; for selected illustrations of these phenotypes see Figure 3. The abnormal embryos were further subdivided into three categories of severity (‡) of the abnormality: mild, moderate or severe, according to the criteria listed in Table 6;**‘Lost’ indicates that embryos were lost during processing (mostly through aspiration during pipetting of buffer or other reagents). Note that 23.7% of all embryos (ethanol and vehicle) were lost by 5 dpf. Very few embryos survived after treatment at the earliest three stages (dome, 50% epiboly and 75% epiboly) with ethanol but all lost. For these reasons, these stages are not analyzed further.