Plant biostimulants treatments in Pisum sativum

Using next-generation plant biostimulants (PBs) could be help to enhance tolerance to abiotic and biotic stresses, vegetable crop quality or nutrient efficiency which is particularly important for vegetable, such as Pisum sativum. The effective extraction methods of herb, the supercritical carbon dioxide (SC-CO2) extraction and the nanoscale drug delivery system as the formulation of PBs offers greener processes and products with preserve bioactive compounds than conventional alternatives. The herbal drug-containing plant biostimulators Elice16Indures and Fitokondi were used with different doses in pea field experiments to monitor the potential of enhancing crop quality and defense responses against different stress factors. Fresh leaves were collected after treatments for QuantSeq 3 mRNA sequencing at Illumina NextSeq 550 platform and libraries were investigated by genome-wide transcriptional profiling focusing on genes associated defense response pathways.