Mitochondrial proteins of T. brucei identified by BioID2 approach.

Column E shows the probability that a given protein has the mitochondrial import signal detected with the Mitofates online prediction tool. Columns F and G display whether a given protein was previously experimentally localized in the mitochondrion (TrypTag) or was present in the Tom40-based depletome. Columns J to Q show enrichment of a given protein in the bait protein datasets compare to IscU_leader_BioID2 negative control dataset. +, statistical significance (Two-sample test) of enrichment in the previous column. Columns R to AF display a Log2-transformed intensities for a given protein in a specific dataset. Lower case letters a, b, and c represent each replicate. Three proteins highlighted in blue were not present in the Tom40-based depletome, nor were they experimentally localized. (XLSX)