The Impact of the CpxA on Regulating Polymyxin B Resistance in Salmonella Typhimurium

Polymyxin B is considered as a last-resort antibiotic for multidrug-resistant or extensively drug-resistant gram-negative bacterial infections. Addressing Salmonella resistance to polymyxin B is crucial for global public health. In this study, transcriptomic detection and analysis were used to clarify the mechanisms by which CpxA-deleted S.typhimurium is involved in resistance to polymyxin B stress, which may be related to processes such as increased assembly of bacterial flagella. Overall design: S.typhimurium and its CpxA-delated strain were inoculated into LB broth containing 0.125 µg/ml polymyxin B, and cultured overnight at 37°C, followed by dilution and subculture. After growing to the logarithmic phase, the bacteria were washed twice with PBS, collected, quickly frozen in liquid nitrogen, and then sent to Beijing Novogene Bioinformatics Technology Co., Ltd. for transcriptome sequencing and analysis.