Gene expression profile at single cell level of non-myocytic cells of the heart (NCM) from TRPM4 WT and KO mice 24h or 72h after SHAM intervention or LAD ligation

Many different cell types constitute the heterogenous group of non-cardiomyocytes (NCM) of the heart. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of NCM and especially how the absence of TRPM4 affects the inflammatory response post myocardial infarction. Overall design: NCMs were isolated from B6.Cg-Trpm4tm1.2-PG (KO) mice or WT mice hearts either 24h after SHAM intervention or LAD ligation or 72h after LAD ligation. The live NCMs were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of Calcein-AM and DAPI signal and the NCM of 4 mice per sample/condition were pooled and analyzed using scRNAseq.