Genome-wide analysis of total, cytoplasmic and nuclear levels of mRNA upon infection of A549 cells with A/WSN/1933 at an MOI of 5 for 6h.. Genome-wide analysis of total, cytoplasmic and nuclear levels of mRNA upon infection of A549 cells with A/WSN/1933 at an MOI of 5 for 6h.
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下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA530925
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资源简介：
RNA of total cell, or nuclear fraction, or cytoplasmic fraction was prepared using RNeasy Plus mini Kit. Purified RNA was quantified by Nanodrop (Thermo Fisher). The RNA samples were subjected to transcriptome analysis using Illumina Human BeadChip 22k according to manufacturer’s instructions. Overall design: A549 Cells are infected with A/WSN/1933 at an MOI of 5 for 6h and total cell extracts and also cytoplasmic and nuclear fractions are collected and subjected to mRNA microarray quantification.
创建时间：
2019-04-04
