Profiling the bone marrow from multiple myeloma patients validates differential expression of AP-1 complex within malignant plasma cells

AP-1 differential expression pattern within plasma cell populations is observed based on single-cell RNA-Seq data analysis. In order to validate this observation and further explore how differences in the AP-1 pathway could lead to biological differences in plasma cell subpopulations, we performed CyTOF experiments with four additional MM patient samples.
Conclusion:
Within plasma cells, distinct clusters with differential expression of JUN and FOS (AP-1 family members) could be observed/
Notes:
1. Samples from four myeloma patients are used for profiling. Among them, case 68043 has a less preferred viability and therefore excluded for downstream analysis. 2. We designed two target panels to separate relevant cell types, quantify signaling pathways (e.g. JAK-STAT, NK-kB), and investigate interleukin activity. 3. Immune profiling is conducted by mass cytometry (CyTOF). Due to the batch effect intrinsic to this technology, downstream processing was done based on batches (labelled by date when the experiment was conducted). Pooling all samples together for analysis may lead to bias. Titration and validation of osmium tetroxide staining for mass cytometry