Constructing a knockout library (BHK-21 GeCKO) using BHK-21 monoclonal cell lines stably expressing Cas9 protein, screening for functional genes necessary for EHV-1 replication, and providing theoretical basis for anti EHV-1 infectio

BHK-21-Cas9 cells were extensively infected using the CRISPR/Cas9 whole genome library; A total of 5767 negative screening genes, including Foxd4, Card 14, Cdk20, and 620 positive screening genes, including Plekhg5, Alox15, Slc35a2, were obtained; GO enrichment analysis revealed that these genes are enriched in the regulation of synaptic vesicle circulation, cell apoptosis, bone marrow cell differentiation, positive regulation of mitochondrial cytochrome c release, molecules related to elastic fibers, negative regulation of glial cell migration, pericyte differentiation, regulation of membrane invagination, chromosome condensation, and other processes.