RNA sequencing analysis of bladder tissue from CFT073-infected C57B/6J mice with or without treatments of D-xyl@epsilonPLCDs and epsilonPLCDs

We designed D-xylose-modified, epsilon-poly-L-lysine-based carbon dots (D-xyl@epsilonPLCDs) to effectively inhibit intracellular UPEC. Treatment with D-xyl@epsilonPLCDs ultimately resulted in a reduction of UPEC in the bladders of mouse UTIs model. To investigate the regulation of immune responses in bladders of CFT073-infected mice post-D-xyl@epsilonPLCDs treatment, RNA sequencing analysis were performed. RNA sequencing was conducted to investigate the regulation of immune response in the bladder of CFT073-infected mice following treatment with D-xyl@epsilonPLCDs. Eight-week-old female C576B/J mice were infected with CFT073 for 6 hours and subsequently treated overnight with either epsilonPLCDs, D-xyl@epsilonPLCDs or sterilized water. The bladders were harvested, rinsed with 75% ethanol, and subjected to RNA extraction using TRIzol methods followed by deep RNA sequencing. Subsequently, we performed gene expression profile analysis based on RNA sequencing data obtained from the bladders of three groups of mice (Control, epsilonPLCDs and D-xyl@epsilonPLCDs), with five mice per group.