Identification of SNPs and Candidate genes for Falling Number of Wheat based on Bulked Segregant RNA-Seq

Ten lines of recombinant inbred line of wheat with extremely high falling number (FN) and ten lines of recombinant inbred line with extremely low FN were chosen. RNA were extracted from grains at 35 days after flowering for each line, then equal amounts of RNA from ten lines with extremely high FN were mixed to high FN bulk (HFN), and equal amounts of RNA from ten lines with extremely low FN were mixed to low FN bulk (LFN). Bulked Segregant RNA-Seq (BSR) were applied and compared between HFN and LFN to identify genes or SNP/InDels related to FN.