Differential microRNA editing may drive target pathway switching in human temporal lobe epilepsy. Differential microRNA editing may drive target pathway switching in human temporal lobe epilepsy

Transcriptional profiling of human-iPSC derived neurons treated with antimir-edited-miR-376a-3p at site +6 (Anta-E), antimir-native-miR-376a-3p (Anta-N) and scramble (Scr) Overall design: Three-condition experiment, Scramble (control) vs. Antamir-E-miR-376a-3p, Scramble (control) vs. Antimir-N-miR-376a-3p, and Antimir-E-miR-376a-3p vs. Antimir-N-miR-376a-3p. Biological replicates: 8 scramble (controls), 8 antagmir-E-miR-376a-3p, and 8 antimir-N-miR-376a-3p . Each biological replicate is human-iPSC derived neurons that have been differentiated for 30 days. Neurons were transfected with 300 nM of scramble, anta-E or anta-N for 48 hours, and later subjected to RNA extraction for transcriptome analysis.