Transcriptomic analysis of loss of Gli1 in neural stem cells responding to demyelination in the mouse brain.

In the adult mammalian brain, Gli1 expressing neural stem cells reside in the subventricular zone and their progeny are recruited to sites of demyelination in the white matter where they regenerate oligodendrocytes, the myelin forming cells. Remarkably, genetic and pharmacologic inhibition of Gli1 enhances remyelination in the adult brain. To understand the molecular mechanisms involved, we performed a transcriptomic analysis to compare the expression of genes in Gli1 neural stem cells with either intact Gli1 expression or genetic loss of Gli1 from adult mice on control diet or cuprizone diet, which induces demyelination. These data will be a valuable resource for identifying therapeutic targets for enhancing remyelination in demyelinating diseases like multiple sclerosis. Overall design: Differential gene expression in fac-sorted fate-mapped Gli1 neural stem cells from the adult subventricular zone of Gli1-Het (Gli1 CreER/wt; Rgfp) and Gli1-null (Gli1 CreER/CreER; Rgfp) mice on control diet vs 0.2% cuprizone diet for 3 weeks. Total 12 samples were analyzed consisting of 3 replicates each from Gli1-Het mice on control diet, Gli1-Het mice on cuprizone diet, Gli1-Null mice on control diet and Gli1-Null mice on cuprizone diet. Grant ID: DOH01-STEM5-2016-00305 Funding source: New York State DOH Grant title: Mobilizing Neural Stem Cells for Remyelination Name: James Salzer