Activity of NOD1 selective compounds and inhibitors of IKK and RIP2 in cell-based assays used for hit identification and triage.

IC50 values are given in micromolar. For each of the cell-based assays, the concentration of IL-8 released into medium was the end-point measured. For the RIPK2 biochemical assay, the level of RIPK2 autophosphorylation was measured. Cell-based assays included are:- Monocyte = human primary peripheral blood monocytes; 293/hNOD1 and 293/hNOD2 = HEK293 cell lines stably expressing full-length human NOD1 or NOD2, respectively; HCT116 = human colon carcinoma cells.