Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by Handmade cloning and in vitro fertilization.

Evaluation of global DNA methylation profiles of cloned embryos at 2-cell, 8-cell, and blastocyst stages and compare it with corresponding stages of embryos produced by in vitro fertilization (IVF) by using MeDIP-Sequencing on Illumina-based platform. It is hypothesized that cloned embryos exhibited significantly different DNA methylation patterns as compared to IVF embryos that could be the reason behind the low blastocyst rate and live birth rate in the case of cloning.