The cytotoxic potential of tolerant CD8+ T cells is not regulated by Nrp1.

WT or Nrp1-deficient CD90.1+ Gag-specific CD8+ T cells were transferred into Alb∶Gag recipients with or without Listeria infection. Three days after transfer, recipients were infused with a 1∶1 ratio of Gag (eFluor 670low) and control (eFluor 670high) peptide-pulsed target cells. Twenty hours later (day 4), recipient spleens were harvested and the frequency of transferred Gag-specific T cells was determined by flow cytometry (upper panels). Inset numbers are the percent of total splenocytes within the inscribed regions. Target cell frequency is displayed as histograms with the percentage of total eFluor 670-positive cells inset above the indicated regions (lower panels). (B) Graph displays relative target cell killing (% eFluor 670high/% eFluor 670low) pooled from 3 independent experiments. Error bars represent standard deviation (ns = not significant).