Injured sensory neurons-derived Galectin-3 contributes to neuropathic pain via programming microglia in the spinal dorsal horn

Gal3 plays an important role in chronic pain through the activation of microglia. We used single nucleus RNA sequencing (snRNA-Seq) to uncover the mechanisms of Gal3-activated microglia in pain regulation. Overall design: Spinal dorsal horn (SDH) from 6-days vehicle or Gal3-treated mice, 3 male mice for each group were used to perform the snRNA-Seq (2 groups: vehicle control (1 sample containing with 3 male mice's SDH) and Gal3-treatment (1 sample containing 3 male mice's SDH)).