Expression data of RPTEC cells treated with/without HGF, aMD5-PEG11 and aMD5-BMH

We synthesized HGF mimic Met-agonist, which is a kind of Met high-affinity dimeric peptide that can activate the Met through dimerization of Met, and induce cell growth, migration, and branching morphogenesis in a similar manner to HGF. Here we detected the gene expression profile induced by HGF and artificial Met-agonist (aMD5-PEG11 and aMD5-BMH). HGF and aMD5-PEG11 show similar induction of gene expression pattern, including multicellular organismal development, multicellular organismal process and anatomical structure development. RPTEC cells were cultured in Cellmatrix type I-A collagen at 3D condition, and treated with/without 1.2nM HGF, 120nM aMD5-PEG11 and 120nM aMD5-BMH for 8 hr. Total RNA was extracted and RNA samples (n=2 for each groups) were subjected to microarray analysis.