Analysis of intestinal microbiota of the kuruma shrimp Penaeus japonicus with different growth rates

This study aimed at investigating the structure and composition of core and signature bacteria in the gut of Penaeus japonicus at different growth rates. We conducted a 7-week feeding experiment, After the experiment, the shrimps were sorted according to their weight and divided into two groups: fast growing and slow growing. And then, we collected the intestinal tissues of the shrimp for intestinal microbiota analysis. The intestinal tissues of every 5 shrimps were one sample, and each experimental group had a total of 3 samples. Total bacterial DNA from all shrimp intestines samples was extracted by the CTAB method. The V4-V5 region of 16S rRNA genes was amplified by PCR. Sequencing libraries were generated using TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA). The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required