Raw data "Fast and accurate quantification of double-strand breaks in microsatellites by digital PCR".

Figure 1: Southern blot original .tif images and quantification of the DSB in the xlsx files (see also Table 1). Figure 1BC-SB annotated.pptx represents the annotated Southern blots. Figure 2: Original image of the Ethidium bromide gel of the DSB-PCR. Image annotated with the main band around 500 bp and with indications of the TALEN ON (+DSB) or OFF (-DSB). Figure 3A: Original qPCR file (.pcrd) for the standard range. Ct results and calculations for the elaboration of the standard range are presented in the .xlsx files. Figure 3BC: Original qPCR files (.pcrd) for the SYBR Green concentration tests. Melting curves and Ct results for each SYBR Green concentration are presented in the .xlsx files. Figure 4CDE: Original dPCR files (.ncx and .ncr - can be opened using CrystalMiner software) for the quantification of the 31% DSB in the CTG repeats (quantified by Southern blot) and the 7% DSB (also quantified by Southern blot). Table 2: Sample 1 (31% DSB) and Sample 2 (7% DSB). xlsx files extracted from the dPCR .ncx file and represent the results obtained for DSB quantification (31% and 7% DSB). The extracted data provide results for the Blue_Channel_Concentration (cp/µL) and Green_Channel_Concentration (cp/µL), which allow us to calculate the percentage of DSB. Only the yellow lines in the xlsx files are used to quantify the DSB (representing 1 ng of DNA used in the dPCR reaction). Supplementary Data Figure 1A: Original qPCR file (.pcrd) for primer efficacy (CP19/upCTG fwd and JEM1 300bp primers used in this study). The xlsx file represents the quantification and calculations of primer efficacy. Supplementary Data Figure 1B: Original dPCR files (.ncx and .ncr - can be opened using CrystalMiner software) for the quantification of the number of positive droplets depending on DNA dilution to assess primer efficacy (CP19/upCTG fwd). The xlsx file represents the quantification and calculation of primer efficacy.