Single cell RNA-seq analysis of endothelial cells during diabetic atherosclerosis. Single cell RNA-seq analysis of endothelial cells during diabetic atherosclerosis

Single-cell RNA sequencing (scRNA-seq) was performed on the CD31-mircrobeads enriched endothelial cells isolated from aorta and heart from eight-week-old, male Klf11f/f-Ldlr-/- and Klf11ECKO-Ldlr-/- (Klf11flf-Tie2Cre-Ldlr-/-) mice fed a diabetogenic high-fat diet with 0.15% cholesterol (DDC) diet for 12 weeks. This study report the changes of EC subpopulations, fractions and transcriptomics during diabetic atherosclerosis. Overall design: Examination of the transcriptomic profiles of endothelial cells isolated from aorta and heart pooled from three Klf11f/f-Ldlr-/- or Klf11ECKO-Ldlr-/- mice fed a diabetogenic diet with 0.15% cholesterol (DDC) diet for 12 weeks