Cell lines constructed for the characterization of T. brucei MIP.
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Cell lines used in this study are based on the parental 29–13 procyclic stage (WT), which stably expresses T7 polymerase and tetracycline repressor, under the resistance cassettes of hygromycin and G418, respectively. Inducible stem-loop RNAi and constitutive expression constructs were targeted to rRNA loci for insertion by homologous recombination. ROI = region of interest.
创建时间:
2018-04-27



