Effect of hBD-2 treatment on gene expression in the colon of acute graft-versus-host disease (GVHD) mice

Wildtype (WT) BALB/c mice were treated with allogeneic hematopoietic cell transplantation (allo-HCT) in order to induce acute GVHD. C57BL/6 mice were used bone marrow (BM) and T cell donor mice. We studied the effect of oral hBD-2 treatment versus vehicle (PBS) control treatment on the gene expression in the colon, as main GVHD target organ. 4 GVHD mice were orally treated with PBS and 4 GVHD mice were orally treated with hBD-2 (1.2 mg/kg) from day 0-7 after allo-HCT. Colon was isolated for RNA isolation on day 7 post allo-HCT