Ringed seal (Phoca hispida) induced multipotent stem cells transcriptome analysis data

We generated ringed seal (Phoca hispida) induced multipotent stem cells from fibroblasts by overexpression of human transgenes: OCT4, SOX2, KLF4 and c-MYC. The resulting cell line, iPHIS1, had the expression of pluripotency marker gene Rex1. Differentiation in embryoid body-like structures allowed us to register expression of AFP, endoderm marker, and Cdx2, trophectoderm marker, but not neuronal (ectoderm) markers. The cells readily differentiated into adipocytes and osteocytes, mesoderm cell types of origin. Transcriptome analysis allowed us to conclude that the cell line does not resemble human pluripotent cells, and, therefore, most probably are not pluripotent. Thus, we produced ringed seal multipotent stem cell line capable of differentiation into adipo- and osteocytes. The raw sequence data are available in NCBI BioProject repository, accession number: PRJNA718133, link: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA718133. The data below represents transcriptome analysis.本研究通过过表达人类转基因因子OCT4、SOX2、KLF4与c-MYC，从环斑海豹（Phoca hispida）成纤维细胞中制备诱导多能干细胞。所得细胞系命名为iPHIS1，其表达多能性标记基因Rex1。在类胚体结构中诱导分化后，可检测到内胚层标记物AFP与滋养外胚层标记物Cdx2的表达，但未检测到神经（外胚层）标记物的表达。该细胞可高效分化为成脂细胞与成骨细胞——二者均为起源于中胚层的细胞类型。转录组分析结果显示，该细胞系的转录谱与人类多能干细胞存在显著差异，因此其大概率不具备多能性。综上，本研究成功构建了可分化为成脂细胞与成骨细胞的环斑海豹多能干细胞系。原始测序数据已上传至NCBI BioProject数据库，登录号为PRJNA718133，访问链接：https://www.ncbi.nlm.nih.gov/bioproject/PRJNA718133。下文所呈现的数据即为该转录组分析结果。