Dexamethasone inhibits Mycobacterium tuberculosis-induced glycolysis but preserves antimicrobial function in primary human macrophages.

This data belongs to the figures from Thong et al 2025 Scientific Reports
(Dexamethasone inhibits Mycobacterium tuberculosis-induced glycolysis but
preserves antimicrobial function in primary human macrophages). The data
is presented in a single excel file. Each tab in the excel file represents
a different figure from the above paper.  It is highly
recommended to read the excel file in conjunction with each figure within
the associated paper. The data has been organised based on the layout of
figures in the associated Scientific Reports paper.  This data
consists of human monocyte derived macrophages and human alveolar
macrophages. Human macrophages were left untreated or treated
with vehicle control (ethanol, 0.02%) or dexamethasone (0.05-5 μM, as
indicated). To model pathways of cellular death, MDM were treated with
staurosporine (1 μM) for 24 h. The effects of dexamethasone on macrophage
metabolism was examined by Seahorse metabolic flux analysis using the
glycolytic rate assays and Mitostress tests (Excel sheet 1 – labelled
Figure 1 hMDM, Excel sheet 2 – labelled Figure 2 hAM, Excel sheet
3 – labelled Figure 3 hMDM).  The expression of mRNA transcripts
of metabolic enzymes were assessed to support the functional metabolic
data (Excel sheet 1 – labelled Figure 1 hMDM, Excel sheet 3 – labelled
Figure 3 hMDM). The ability of dexamethasone to alter the secretion of
cytokines IL-1β, TNF, IL-6, IL-8 and IL-10 was assessed in supernatants
from uninfected and Mtb infected (H37Ra) macrophages (Excel Sheet 4 –
labelled Figure 4 hMDM). The effect of dexamethasone cell death and
caspase activation in macrophages was examined (Excel Sheet 5 – labelled
Figure 5 hMDM). Finally bacterial control of macrophages was examined in
dexamethasone treated macrophages in the presence of  bafilomycin
or rapamycin. Bafilomycin A1 (50 nM) or rapamycin (250 ng/ml) were
used  to examine the role of autophagy in dexamethasone induced
bacterial control (Excel Sheet 6 – labelled Figure 6 hMDM). These
data were calculated by assessing colony forming units assays on
Middlebrook agar. Supplemental Figures 1 and 2 are supporting data for
Seahorse Assays (Excel Sheet 7- labelled Figure S1 
hMDM, Excel Sheet 8- labelled Figure S2
hMDM). Supplemental Figure 3 is supporting data for bacterial
growth assays demonstrating phagocytosis on day 0 (Excel Sheet 9- labelled
Figure S3 hMDM).