Liver Cell Culture on ITO-SAMs and Metabolite Analysis using NMR: Monomers for Biological Macromolecules and Lipoprotein

NMR spectroscopy provides valuable metabolomic data for liver toxicity screening using HepG2 cells cultured on self-assembled monolayers. This approach offers a comprehensive view of intracellular metabolites, enabling the detection of subtle biochemical changes induced by potential hepatotoxic compounds while focusing on key details like sensitivity, reproducibility and metabolomic profile for drug screening and toxicology applications.核磁共振波谱法（NMR spectroscopy）可为在自组装单分子层上培养的HepG2细胞的肝脏毒性筛选提供极具价值的代谢组学数据。该方法可全面表征细胞内代谢物，实现对潜在肝毒性化合物诱导的细微生化变化的检测，同时兼顾药物筛选与毒理学应用中灵敏度、重现性及代谢组学图谱等关键细节。