Total RNA sequencing of prospective axial stem cell cultures derived from human pluripotant stem cells

Here we describe a protocol for converting pluripotent stem cells to stable bi/multipotent stem cell populations with axial progenitor characteristics. To analyse the global transcriptome of the derived lines, we performed RNA-sequencing of 3 different axial stem cell lines Overall design: total RNA sequencing of three stable cell lines at passage 9 and undifferentiated parental hESC line H9