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Regulation of gene expression by STIM1 and Ca2+ in human T cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE179625
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Human CD4+ T cells were left untreated or stimulated with 5 μg/ml plate-bound anti-CD3 antibody (clone OKT3, 14-0037-82, eBioscience) and 10 μg/ml soluble anti-CD28 antibody (clone CD28.2, 14-0289-82, eBioscience) for 6 h in RPMI1640 medium supplemented with 10% FBS, 1% L-glutamine, and 1% penicillin-streptomycin. RNA Sequencing of human CD4+ T cells from a patient homozygous for a STIM1 c.497+776A>G mutation, heterozygous carriers and a healthy donor
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2022-05-04
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