Downregulation of MED12 via siRNA in 22Rv1 prostate cancer cells (full transcriptome)

The Mediator complex is a multi-subunit protein that modulates gene expression on a genome-wide scale. MED12 and cyclin-dependent kinase 8 (CDK8) or its paralog CDK19 are components of its kinase module that regulate the proliferation of prostate cancer cells. In this study, we investigated how MED12 and CDK8/19 influence cancer-driven processes in prostate cancer cell lines, focusing on AR activity and enzalutamide resistance. To investigate the relevance of MED12 in de novo enzalutamide resistant 22Rv1 cells, we performed siRNA mediated knockdown and analyzed the response using differential gene expression analysis.