AFM experiments on lipid bilayers

Raw AFM topography images of DPPC:POPC (1:1 by mol) supported on mica lipid bilayer before (named AFM_DPPCPOPC_5um.spm) and after (named AFM_DPPCPOPC_BR_1660nm.spm and AFM_DPPCPOPC_BR_800nm.spm) bacteriorhodopsin insertion are provided. DPPC:POPC (1:1 by mol) supported lipid bilayer was formed by vesicles fusion. BR reconstitution was performed by injection a solution of 1 microg/ml of BR in 0.05 mM DDM in PBS buffer, incubating system for 15 min and extensively rinsing with buffer afterwards. The sample imaging was performed at room temperature in Peak Force Tapping mode, with the force set point value of ~ 300 pN at a scan frequency of a 1-2 Hz. Raw AFM image of BR deposited on POPC/DPPC/mica surface (named AFM_BR_600nm.spm) is provided. The image only shows BR deposited on mica with their symmetry axis perpendicular to the surface. A membrane edge can be observed at the very bottom left of the scan. The image is made of 1024 x 1024 px$^2$ collected at 1 Hz using the Peak-Force Tapping mode in a semi-automatic control.本数据集提供了负载于云母基底的DPPC:POPC（摩尔比1:1）支撑磷脂双层膜在细菌视紫红质（bacteriorhodopsin）插入前后的原始原子力显微镜（Atomic Force Microscopy，AFM）形貌图像：其中插入前的图像命名为AFM_DPPCPOPC_5um.spm，插入后的两张图像分别命名为AFM_DPPCPOPC_BR_1660nm.spm与AFM_DPPCPOPC_BR_800nm.spm。该DPPC:POPC（摩尔比1:1）支撑磷脂双层膜通过囊泡融合法制备。细菌视紫红质（BR）的重组过程为：将浓度为1 μg/mL的BR溶液（溶剂为含0.05 mM十二烷基β-D-麦芽糖苷（DDM）的磷酸盐缓冲液（PBS））注入体系，孵育15分钟后用缓冲液充分漂洗。样品成像在室温下采用峰值力轻敲模式（Peak Force Tapping mode）完成，力设定点约为300皮牛（pN），扫描频率为1~2 Hz。本数据集还提供了一张沉积于POPC/DPPC/云母表面的BR原始AFM图像，其文件名为AFM_BR_600nm.spm。该图像仅显示了沉积于云母表面的BR，其对称轴垂直于基底表面。在扫描区域的最左下角可观察到膜边缘。该图像分辨率为1024×1024像素，以1 Hz的采集速率通过半自动化控制的峰值力轻敲模式采集得到。